These are usually not too mathematically complex and are a good guide as to what to do practically. Biomolecular NMR Spectroscopy. Jeremy N. Evans OUP. This was an excellent textbook when it came out but some of it is starting to be dated. Goes beyond the level of understanding of NMR tehory required for the course.
Fairbrother , Arthur G. Again much more detailed than required for the course.
David Blow was one of the pioneers of crystallography and this is a very well written book. Probably the best of these selections Biomolecular Crystallography Bernhard Rupp Next you will inspect other ambiguous positions in your sequence. Move the cursor to each base designated N.
Inspect the chromatogram at this position. It is frequently possible to guess the correct base directly from the chromatogram picture.
Change N to your guessed base by typing the letter. Save the sequencing project. In order to screen for vector contamination, you must specify the vector used for amplifying your fragment. To enter or load vector informa- tion perform the following. Choose the trim vector command from the sequence menu.
The vec- tor contamination window will open. The vector insertion site window will open. When the polylinker window is displayed, click the site where your fragment was in- serted into the vector. Since we cloned our insert into SmaI sites, highlight it and click the OK button.
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Save this vector by clicking on the save sites button top of the window. The computer searches the selected fragments for overlap with the vector bases entered. Any of the searched fragments that are found to contain vector bases will be displayed in the vector screening dialog box. The window will show your sequence as a single line in two colors.
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Blue color indicates your sequence, whereas vector sequences are red. A scissor icon will separate the sequences. The SmaI site should be displayed on the border between the vector and insert. Perform this analysis with all of your sequences and save the project again. References Altschul, S.
Altschul, S. Nature Genet.
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